Volume 55
Use of Biochemical and Molecular Genetics Techniques to Distinguish Two Closely Related and Moxphologically Similar Carcharinid Shark Species-Carcharhinus brevipinna and Carcharhinus limbatus
Authors
Blandon, I.R.; Ward, R.; Karel, W.J.; Mobley, B. Download PDF Open PDF in BrowserOther Information
Date: 2004
Pages: 1015
Event: Proceedings of the Fifty Fifth Annual Gulf and Caribbean Fisheries Institute
City: Xel Ha
Country: Mexico
Abstract
Species identification for closely related, morphologically similar shark species is difficult under field conditions or when using processed samples. Various molecular markers have been utilized to distinguish among easily misidentified sharks. Current research at Perry R Bass Marine Fisheries Research Station has required the examination of blacktip shark fin clips obtained from diverse localities. Some samples were collected by local fishermen. Due to the possibility of confusing blacktip sharks with spinner sharks the utility ofvarious protein and DNA markers have been explored to distinguish between these species. Isoelectric focusing (IEF) and sequencing of PCR amplified nuclear fragments were investigated.