Volume 52

Determinación Electroforetica del Patron Proteico de Tres Poblaciones del Carocol Rosado, Strombus gigas, en las Costas de Quintana Roo y el Uso de un Software de Imágenes para su Analisis


Authors
Tello-Cetina, J.A.; Rodríguez-Gil, L.A.
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Date: November, 1999


Pages: 184-193


Event: Proceedings of the Fifty Second Annual Gulf and Caribbean Fisheries Institute


City: Key West, Florida


Country: USA

Abstract

The determination of the proteinogram of samples of muscle of the pink snail, Strombus gigas, obtained in Isla Mujeres, Punta Allen and Banco Chinchorro in the north area, center and south of Quintana Roo's state, was carried out by means of the electrophoresis technique in acrylamyde gels (pAGE) to 10% between disociante (SDS) with the purpose of establishing the possible geographical differentiation between populations.\The visual analysis of the proteinograms indicated similarity in the number of proteins fraction for samples taken from Punta Allen and Isla Mujeres and differences between these places and Banco Chinchorro. However, when the proteinograms were subjected to digital analysis (PROANA), the difference settled down among males and female was determined 12 fractions for the males and 13 fractions for t females in Punta Allen and Isla Mujeres and of 15 and 16 fractions for males and females in Banco Chinchorro.\The results of this work provide an evaluation of the efficacy of computer software ( PROANA) as a tool for digital analysis of protein fractions revealed in proteinograms that cannot be detected visually, besides reducing the costs and times of work in the analysis.\The possible geographical separation of the populations of Strombus gigas in the Mexican Caribbean, with the reservations that the used method presents, should be considered in the interpretation of the results reported in this study.

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