Volume 52

Induction of Ovulation in Common Snook, Centropomis undecimalis (Bloch), using Human Gonadotropin (HCG) and Gonadotropin-Releasing Hormone (GNRH)

Neidig, C.L.; Skapura, D.S.; Grier, H.J.
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Date: November, 1999

Pages: 360-362

Event: Proceedings of the Fifty Second Annual Gulf and Caribbean Fisheries Institute

City: Key West, Florida

Country: USA


Common snook, Centropomus undecimalis, is one of the Florida's most popular inshore sport fish and the subject of an ongoing stock enhancement program. In the development of hatchery techniques for spawning this species, both human chorionic gonadotropin (HCG) and gonadotropin-releasing hormones (GnRH) were investigated. Doses of HCG were compared to determine the smallest dose that would induce ovulation and produce optimum egg and larval survival. Doses of 50, 100, 250, 500, 1000, and 2000 IU/kg body weight (BW) were used. A dose of 500 IU/kg BW of HCG produced consistent ovulation, good egg quality and larval survival. Secondly, four analogs of GnRH were each administered in time-release pellets at a dose of 10 µgm/kg/day over five days. These were salmon (sGnRH), chicken (cGnRH-II), seabream (sbGnRH), and mammalian (mGnRH) gonadotropin-releasing hormones. With the exception of sbGnRH which was ineffective, the time to ovulation was similar with all hormones, and viable larvae were obtained. Control fish did not ovulate.

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